Managing Acetate Accumulation in Recombinant E. coli Fed-Batch Processes

This is particularly with recombinant Escherichia coli strains. It
draws from a real-world case study of unexpected acetate, lactate, glycerol, or organic acid
accumulation during fed-batch operations, caused by feed strategy misalignment with cellular uptake
kinetics. The focus is on a recombinant E. coli vaccine antigen process that failed GMP batches due to
acetate toxicity at pilot scale